#Spectral flow cytometry full

The Hugh Green Cytometry Centre are leaders in the field of full spectrum flow cytometry and have published several papers in well-known journals. Three of our four Auroras are equipped with plate loaders to allow for high-throughput sample acquisition.

There are four Auroras housed at MIMR, two with a 5-laser configuration (UV, Violet, Blue, Yellow-Green and Red), and two with a 4-laser configuration (Violet, Blue, Yellow-Green and Red). The Cytek Aurora is a full spectrum flow cytometer which can resolve 40+ colours in a single panel. It is also possible to use AF to identify and quantify unlabelled populations based on their autofluorescent signatures (e.g., different phytoplankton populations).įull spectrum flow cytometry enables high-throughput characterisation of 40+ parameters at the single cell level, which gives researchers greatly increased flexibility in panel design and allowing for the generation of high-dimensional immunophenotyping panels of a size only before seen with mass cytometry. Full spectrum flow cytometry makes it easy to identify and account for AF in any tissue type.

In flow cytometry AF creates background that can impair the detection of dim markers emitting light at the same wavelengths andcan attribute to false positives if not accounted for. One of the extraordinary powers of spectral cytometry is the ability to capture the autofluorescence (AF) signature of different cells types or entities within a sample and to use these as unique markers for identification, and/or for specific removal during unmixing.ĪF is an intrinsic characteristic of cells caused by the presence of fluorescent biological compounds within the cell these can include lipofuscin, riboflavin and flavin coenzymes, structural proteins, metabolites and cellular organelles.

Spectral flow cytometry